| http://www.w3.org/ns/prov#value | - where Tm is a melting temperature, Wtm is the weight assigned to the Tm (0.3), Tmavg is the average Tm of the two primers, Tmopt is the optimum Tm, Wgc is the weight assigned to GC content (0.1), GCavg is the average percent GC content of the primers, GCopt is the optimum GC content, Wblast is the weight assigned to the blastn alignment (0.3), BlastLength is the length of the longest blastn alignm
|