| http://www.w3.org/ns/prov#value | - Wells were washed three times with washing buffer (Phosphate-buffered saline solution containing 0.05% Tween20, hereafter called PBS-T), and blocked with 1% Bovine Serum Albumin (BSA) in PBS-T (300 ??l per well) for one hour at 37??C. To ensure that there was no interaction between the conjugate and the blocking reagent, and to verify that a 1% solution was an optimal blocking concentration, contr
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