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To increase the infectivity of the bacteria, Agrobacterium is cultured in low-pH induction medium, i.e., any bacterium culture media with a pH value adjusted to from 4.5 to 6.0, most preferably about 5.2, and at low temperature such as for example about 19-30?? C., preferably about 21-26?? C. The conditions of low-pH and low temperature are among the well-defined critical factors for inducing viru
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