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The technique of differential display involves isolation of undegraded MRNA free of genomic DNA. Reverse transcriptase (RT) is necessary forconversion of mRNA to single stranded cDNA by using a two base-anchored oligo-dT primer T12MA, T12MC, T12MG and T12MT where M is a mixture of dA, dC and dG obtained from Beckman Coulter, Calif.
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