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The same volume (10 ??l) of samples was applied to each well of a PAGE gel. (B) The same sample set was used for WBs using anti-FimA, Rgp, HBP35, and A-LPS. In the WB of Rgp, diffuse bands around 60???70 kDa and relatively sharp bands around 40 kDa are observed, probably due to the presence of both glycosylated and non-glycosylated forms of Rgps in the MVs, respectively.
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