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  • So my question is if the kozak sequence is 100% necessary to have a high expression?If it is, is it possible to make a longer primer so that I add a kozak 5' to the ATG of my gene? (I know kozak already contains an ATG so that would make two start codons in a very small distance, but the first part of my gene just is a signal peptide, so maybe it doens't mather too much to make that peptide a litt
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