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  • For example, if 10 ul of a 5x diluted sample is used, then V=0.01 ml and Dilution factor =5.If 25 ul of test sample is added in each well what how much and what solution should be used for parallel background control - Water/Assay buffer?The background control is to account for any engogeous sample-related interference and hence if you are adding 25 ul of sample, you should add the same volume of
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