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  • Strains (segregants) are then preferably isolated which are deficient in proteases A and B and possibly D and in the carboxypeptidases Y and S and, in addition, are auxotrophic for at least one of, e.g. uracil, leucine and maltoseutilization or both leucine and tryptophan utilization or, finally, all of the leucine, uracil and histidine auxotrophies of the parent strains.Therefore, in a further pr
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