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  • Adding the extra protein by doubling the FBS helps to cushion and protect the fragile cell membranes until they can recover. -miBunny- Thanks for that, I have another problem now it looks like 50% of my THP-1s are sticking to the bottom of the flask - is this spontaneous differentiation and what are the causes other than overcrowding which I dont think was the problem unless my cell counts are w
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