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  • The four potential apg9ts colonies were analyzed by pulse/chase labeling to assess the kinetics of prAPI delivery to the vacuole at permissive and nonpermissive temperatures.Membrane Flotation AnalysisThe procedure for the membrane flotation experiments is a modification of a previously described protocol (Scott and Klionsky 1995; Babst et al. 1997; Kim et al. 1999).
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