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  • The above procedure was repeated for ammonia, except cells were diluted in a 1:10 ratio in Peptone-Meat broth (PM; per 1 liter: 5 g peptone and 3 g beef extract and at a pH of 6.5), and, instead of hydroxylamine, 50 ??l of ammonium chloride (400 ??g/ml in PBS, filter sterile) were added to 50 ??l of each strain, providing 52 ppm ammonia-N concentration.
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