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  • Pitfalls include inconsistent time to tissue paraffin embedding and fixation time, uneven antibody epitope access among native in situ versus denatured ex vivo protein forms, polyclonal antibody pools of variable affinity, non-specific and non-reproducible antibody clones from one lot to another, and discordant staining localization such as the case of a predominately cytoplasmic staining when a t
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