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  • reto a salt such as potassium bromide and to about 7.0 in pH, subjected to centrifugation at 4???-25??? C., preferably 4???-15??? C., for 24-40 hours at 100,000 g-200,000 g (centrifugation procedure IV), and a fraction floating to the surface (fraction IV) is collected to obtain endothelin converting enzyme IV.
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