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  • Briefly, a protected lysine-biotin conjugate (or other similar biotin derivative, such as glutamic acid biocytinamide or the like) is prepared; DTPA anhydride is opened with the lysine-biotin (or other derivative); the lysine (or other reactive moiety) is deprotected; the amine (or other functional group) is acylated with an active ester chelate, such as tetrafluorophenyl esters, N-hydroxysuccinim
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