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  • -** The substrate concentration that is used in enzyme assays is chosen according to parameters such as the Km, the solubility of the substrate, whether high concentrations may inhibit, and the cost of the substrate. [http://www.life.illinois.edu/biochem/455/Lab%20exercises/B-gal/enzymology.pdf reference]+* The substrate concentration that is used in enzyme assays is chosen according to parameters
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