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  • The elution profile was: 20% B for 10 minutes, followed by a linear gradient of 20% B to 50% B over 50 minutes, 50% B for 10 minutes, a linear gradient of 50% B to 100% B over 10 minutes, and a linear gradient of 100% B to 0% B over 10 minutes. [0087] The second step was a purification of a completely deprotected, i.e. following the removal of the 5???-DMT group, ribozyme by a treatment with 2% tr
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