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  • In general, the amount of polypeptide that is sufficient for the evaluation of about 105 cells ranges from about 10 ng/mL to about 100 ??g/mL and preferably is about 10 ??g/mL. The polypeptide may be, but need not be, immobilized on a solid support, such as a bead or a biodegradable microsphere, such as those described in U.S. Pat. Nos. 4,897,268 and 5,075,109.
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