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  • In certain embodiments, the addition of a dideoxynucleotide base 36 to the primer 30 is performed using a thermostable DNA polymerase, for example Taq polymerase, AmpliTaq FS or a Taq DNA polymerase modified at position 667, Thermo Sequence???, or alternatively by using an ordinary thermostable DNA polymerase, such as Klenow, T7, or Sequenase DNA polymerase.
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