midate linkage at the terminal phosphate of a natural or modified nucleotide. [0094] In another aspect of the methods of detecting the presence of a nucleic acid sequence described above, the terminal-phosphate-labeled nucleotide may be represented by the Formula I: [0095] wherein P=phosphate (PO3) and derivatives thereof, n is 2 or greater; Y is an oxygen or sulfur atom; B is a nitrogen-containin