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  • Indeed crystals soaked in 2.4 M sodium malonate (which is the lowest concentration that provides cryoprotection at pH 7.0) or in lower amounts (i.e. the concentration of the mother liquor supplemented with other cryoprotectants such as glycerol and ethylene glycol) tended to split.We then attempted the counter-diffusion method using the capillary technique (Garc??a-Ruiz & M
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