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  • Specific recombinant cytokine combinations added to culture in some instances (e.g., rhlL-12, rhGM-CSF, rhIL-4 and rhIL-2, gamma-interferon, TNF-alpha) as well as agents such as endotoxin or amphotericin B.9. Cultured/activated, antigen pulsed APCs harvested using Ca++ /Mg++ free medium (HBSS or PBS) with collection into ice cold buffer in tubes kept on ice.10.
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