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  • For testing the role of DNA methylation we used dam-, dcm-ER2925 E. coli from New England Biolabs (ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 hsdR2).MIC determinationA 2 ml LB culture was inoculated from frozen stocks for each of the cell types and grown to log phase.
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