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  • Stable binding of polynucleotides to other polynucleotides via hybridization is also affected by temperature, salt concentration, nucleotide sequence, and other factors, as has been extensively discussed in the literature; see, e.g., Sanger (1984) in Principles of Nucleic Acid Structure: Chapter 6, pp. 116-158 (Springer-Verlag: New York, USA).
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