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  • After sufficient time to ensure thioacylation of the polypeptide, for a few minutes up to several hours and generally 15 to 45 minutes, the excess reagent is removed by washing of an immobilized polypeptide, and byproducts of the reaction resulting from the decomposition of the reagent are similarly removed, to yield a thioacyl polypeptide of general formula IV wherein X is an alkyl, aryl or aryl-
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