| http://www.w3.org/ns/prov#value | - The apparatus of claim 15, wherein the actuator is disposed within the reagent chamber, the actuator including a plunger and a protrusion, the plunger configured to move a reagent from the reagent chamber into the first chamber when the actuator is moved, the protrusion configured to puncture the portion of the puncturable member when the actuator is moved.
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