| http://www.w3.org/ns/prov#value | - However, I know my protein is an enzyme that resides in the trans Golgi there are not any articles that suggest that its is associated with the phospholipid membranes.I am currently taking a 1 million cell pellet, washed to remove serum, and adding 300 ul of RIPA buffer with protease inhibitors and letting in sit on ice for 30 minutes vortexing two are three times during that time, and then store
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