| http://www.w3.org/ns/prov#value | - Aliquots of the culture medium, sonicated cells and filtrate are taken for analysis, to ascertain whether peptide is secreted or retained in the cell or present as a soluble intracellular/periplasmic protein. [0108] The protein samples are separated using standard polyacrylamide gel elctrophoesis, using a 15%-20% polyacrylamide gel.
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