| http://www.w3.org/ns/prov#value | - i think you should use protease inhibitor with every cell lysis. all kinds of proteases will be released and they will eat your protein. use a tablet from Roche, it is so convenient. about the method, it depends on your protein. if you get good amount of it in the soluble fraction by sonication in PBS let's say you are the luckiest guy on earth! sonication is cheap, quick and always available. if t
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