| http://www.w3.org/ns/prov#value | - Of course, sequencing shows no insert presence..I have tried reducing the amount or DNA used for transformation, but then I get no colonies whatsoever.I do not know if bacteria are particularly unhappy with my plasmid?!They should be supercompetent, at least the Dh5alpha I have been using...I have had no problems with my previous cloning using the different vector in the same bacteria.pleeeease heeelp!
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