| http://www.w3.org/ns/prov#value | - For example, for His-tagged proteins use saturated binding to a Ni sepharose resin and elution with a minimal amount of buffer to concentrate the protein without increasing the detergent content, and finally use the largest possible cutoff centrifugal concentrators.2Protein can be labeled with a variety of fluorescent dyes, readily available from Invitrogen, GE Healthcare and other companies.
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