| http://www.w3.org/ns/prov#value | - Cells such as A549 cells, or neurons are seeded, for example, at 1???105 cells per well of a six-well dish in EGM-2 (BioWhittaker) the day before transfection. siNA (final concentration, for example 20 nM) and cationic lipid (e.g., final concentration 2 ??g/ml) are complexed in EGM basal media (Bio Whittaker) at 37??? C. for 30 minutes in polystyrene tubes.
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