| http://www.w3.org/ns/prov#value | - Typically, the above methods involve some form of enzyme purification, such as His-tag affinity binding, prior to the assay to remove phosphate and other small molecules, as well as other enzymes which can interfere or contribute to background. [0132] In the case of nucleotide analogues labeled with fluorophores (or radio-isotopes), incorporation of the fluorophore (or isotope)-labeled nucleotide
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