About: http://webisa.webdatacommons.org/201104198

Property	Value
http://www.w3.org/1999/02/22-rdf-syntax-ns#type	http://www.w3.org/ns/prov#Entity
http://www.w3.org/ns/prov#value	The only thing I can advise is the usual PCR protocol modifications (which I am sure you have been through), possibly additives such as betaine and to have a back up plan using one of the other mutagenesis procedures.
http://www.w3.org/ns/prov#wasQuotedFrom	protocol-online.org