| http://www.w3.org/ns/prov#value | - ideally you should replace with fresh media and not do the top up thing, it would depend on your cell type, on some sensitive cells leaving the lipid reagent would make the cells unhappy and plus you wouldnt want tiny lipid particles floating about in your culture media which might scare the life out of you if you end up thinking that its some sort of contamination!
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