| http://www.w3.org/ns/prov#value | - use an antibody directed to the C-terminus (and a C-terminal fusion tag if you can...that way, you would only be purifying the full-length fusion proteins). if this is protein is expressed in something other than e. coli, it might be getting proteolytically degraded, either in vivo, or in vitro, depending on the presence/absence of your proteinase inhibitors in your extraction buffer.good luckQUOTE (
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