| http://www.w3.org/ns/prov#value | - Hi, the vector looks good.For the insert after doing PCR, you can run 3ul on a gel and see if it has the band of your interest and if there is no additional band other than that you can just treat it with DpnI to get reed of the template, column purify it, do restriction and then column purify again , that would be good.You do not need to gel purify everytime.
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