| http://www.w3.org/ns/prov#value | - Hi.I have a problem with expression of a protein which has second residue exactly what I want to express.I need to exrpress Mat-Lys~ and Mat-Asn with pAcycDuet vector which has His tag in MSC.Because I want to delete His tag, I have no choice except Nco1 for RE.Also. it is very important to express Lys or Asn as a seond residue in my protein.Here is a problem!Nco1 has ATG start codon in the restri
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