| http://www.w3.org/ns/prov#value | - IFN-?? and IL-4 mRNA levels were measured by real-time PCR to confirm the proper polarization of the Th1 and Th2 cells, respectively (unpublished data).Figure 1.Tpl2 is an IL-12???inducible gene in human and mouse cells. (A, left) Human PBMC were cultured for 3 d in RPMI containing 1 ??g/ml PHA followed by an additional day of culture in 40 U/ml IL-2 to maximize expression of IL-12R (43).
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