| http://www.w3.org/ns/prov#value | - An example of moderately stringent conditions is a condition such as overnight incubation at 37??? C. in a solution comprising: 20% formamide, 5???SSC (150 mM NaCl, 15 mM trisodium citrate), 50 mM sodium phosphate (pH 7.6), 5???Denhardt's solution, 10% dextran sulfate, and 20 mg/mL denatured sheared salmon sperm DNA, followed by washing the filters in 1???SSC at about 37-???50??? C. The skilled ar
|
