| http://www.w3.org/ns/prov#value | - An example of moderately stringent conditions is a condition such as overnight incubation at 37?? C. in a solution comprising: 20% formamide, 5??SSC (150 mM NaCl, 15 mM trisodium citrate), 50 mM sodium phosphate (pH 7.6), 5?? Denhardt's solution, 10% dextran sulfate, and 20 mg/mL denatured sheared salmon sperm DNA, followed by washing the filters in 1??SSC at about 37-50?? C. The skilled artisan w
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