| http://www.w3.org/ns/prov#value | - in of replication and bacterial selection marker are located between the two LTRs.Further provided herein is a method of producing a single-LTR circular HIV-1 form plasmid, comprising: introducing a shuttle vector comprising a nucleic acid of the present invention into a eukaryotic cell; extracting non-integrated DNA from theeukaryotic cell; transforming a bacterial cell with the extracted non-int
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