| http://www.w3.org/ns/prov#value | - Preferably, a fluid applied to the spreading layer, passes through the sample aperture and contacts the membrane. [0011] A second aspect of the present invention is a method of determining the presence or concentration of a target in a sample that includes the steps of: a) applying a fluid suspected of including a target to the reagent test strip of the present invention; b) detecting the reflecta
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