| http://www.w3.org/ns/prov#value | - This should give you much better yield.Here is an example of a forward primer I designed and used to put an HA tag onto a gene:AGCTCGGATCCACCATGTACCCATACGATCGGCCACATTACGCTGCCAAGCATCTCATCACTCTGoverhang-BamHI start ------------HA tag--------------------- gene specific You must include an overhang so your restriction digest will work, some enzymes require more or less.
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