| http://www.w3.org/ns/prov#value | - Proteins produced by E-coli for example, are first isolated from inclusion bodies, and then solubilized using a denaturant or chaotropic agent such as guanidine HCl or urea, preferably in the range of about 4-9 M and at an elevated temperature (e.g., 25-37??? C.) and/or basic pH (8-10).
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