| http://www.w3.org/ns/prov#value | - However, medium is not an ideal buffer for sorting because pH indicators such as phenol red can cause emission interference and calcium in the medium can be precipitated by phosphates in buffers used in the sorting machine.A basic sorting buffer is cation-free PBS with 1 mM EDTA containing 25 mM HEPES at pH 7 and 1% serum.3.
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