About: http://webisa.webdatacommons.org/335822804

Property	Value
http://www.w3.org/1999/02/22-rdf-syntax-ns#type	http://www.w3.org/ns/prov#Entity
http://www.w3.org/ns/prov#value	Are you sure you have not contaminations?If not, try to add 10% DMSO and i also do the hot start before the reaction: 5' at 80???C with all reagents except taq and template, then you will add template and taq -mauro-
http://www.w3.org/ns/prov#wasQuotedFrom	protocol-online.org