| http://www.w3.org/ns/prov#value | - However, cycle sequencing is a variation of Sanger sequencing and many people still refer to this as Sanger sequencing to distinguish it from the new multiplex sequencing methods like 454 sequencing which are based on a different principle. <br>+The cycling reaction starts by denaturing your sample plasmid and annealing the oligo to its homologous sequence.
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