| http://www.w3.org/ns/prov#value | - Skeletal muscles and other organs were isolated and cleaned of fat and connective tissue before being weighed and frozen in liquid nitrogen.Soleus muscles were pulverised in liquid nitrogen then homogenised on ice in 8 volumes of 1% Triton X-100, 50 mM Tris pH 7.4 containing Complete??? protease inhibitor (Roche Diagnostics, Lewes, UK).
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