| http://www.w3.org/ns/prov#value | - Generally the 3??? terminus of the probe will be ???blocked??? to prohibit incorporation of the probe into a primer extension product. ???Blocking??? can be achieved by using non-complementary bases or by adding a chemical moiety such as biotin or a phosphate group to the 3??? hydroxyl of the last nucleotide, which may, depending upon the selected moiety, serve a dual purpose by also acting as a l
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