| http://www.w3.org/ns/prov#value | - Cells were plated at 1???105/ml in 96-well plates in 100 ??l MEM, 10 mM HEPES pH 7.5,0.2% FBS. Following 4 hr incubation at 37??? C., when cells were well attached to the well surface, TGF??1 was added at 1-4 pM with or without nucleic acid ligands as follows: the ligands were diluted across the 96 well plate in 3-fold dilution steps and then TGF??1 was added at 1-4 pM to all wells except controls
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